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Protocoles de virologie végétale : de l'isolement viral à la résistance transgénique par Gary -
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- Achat immédiatProtocoles de virologie végétale : de l'isolement viral à la résistance transgénique par Gary
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Numéro de l'objet eBay :395157027961
Dernière mise à jour le 22 sept. 2024 03:36:58 Paris. Afficher toutes les modificationsAfficher toutes les modifications
Caractéristiques de l'objet
- État
- ISBN-13
- 9780896033856
- Book Title
- Plant Virology Protocols
- ISBN
- 9780896033856
- Subject Area
- Science
- Publication Name
- Plant Virology Protocols : from Virus Isolation to Transgenic Resistance
- Publisher
- Humana Press
- Item Length
- 9.3 in
- Subject
- Life Sciences / Botany
- Publication Year
- 1998
- Series
- Methods in Molecular Biology Ser.
- Type
- Textbook
- Format
- Hardcover
- Language
- English
- Item Height
- 0.5 in
- Item Weight
- 35.3 Oz
- Item Width
- 6.1 in
- Number of Pages
- Xv, 571 Pages
À propos de ce produit
Product Identifiers
Publisher
Humana Press
ISBN-10
0896033856
ISBN-13
9780896033856
eBay Product ID (ePID)
1047974
Product Key Features
Number of Pages
Xv, 571 Pages
Publication Name
Plant Virology Protocols : from Virus Isolation to Transgenic Resistance
Language
English
Publication Year
1998
Subject
Life Sciences / Botany
Type
Textbook
Subject Area
Science
Series
Methods in Molecular Biology Ser.
Format
Hardcover
Dimensions
Item Height
0.5 in
Item Weight
35.3 Oz
Item Length
9.3 in
Item Width
6.1 in
Additional Product Features
Intended Audience
Scholarly & Professional
LCCN
97-032185
Dewey Edition
21
Series Volume Number
81
Number of Volumes
1 vol.
Illustrated
Yes
Dewey Decimal
579.2/8
Table Of Content
to Plant Virology.- to Classical Crossprotection.- History of Coat Protein-Mediated Protection.- Virus Isolation, RNA Extraction, and Location of Coat Protein Genes.- Geminivirus Isolation and DNA Extraction.- Caulimovirus Isolation and DNA Extraction.- Reovirus Isolation and RNA Extraction.- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction.- Hordeivirus Isolation and RNA Extraction.- Furovirus Isolation and RNA Extraction.- Tobravirus Isolation and RNA Extraction.- Tobamovirus Isolation and RNA Extraction.- Potexvirus Isolation and RNA Extraction.- Carlavirus Isolation and RNA Extraction.- Potyvirus Isolation and RNA Extraction.- Trichovirus Isolation and RNA Extraction.- Ilarvirus Isolation and RNA Extraction.- Bromovirus Isolation and RNA Extraction.- Cucumovirus Isolation and RNA Extraction.- Nepovirus Isolation and RNA Extraction.- Comovirus Isolation and RNA Extraction.- Carmovirus Isolation and RNA Extraction.- Tymovirus Isolation and Genomic RNA Extraction.- Tombusvirus Isolation and RNA Extraction.- Luteovirus Isolation and RNA Extraction.- Cloning of Coat Protein Genes.- RNA Analysis.- RNA Fractionation by Density Gradient Centrifugation.- cDNA Library Construction for the Lambda ZAP®-Based Vectors.- PCR Cloning of Coat Protein Genes.- Antibody Production.- Expression Library Screening.- In Vitro Transcription and Translation.- Analysis of Coat Protein Expression Cassettes in Protoplasts.- DNA Sequencing.- Computer Analysis of Amino Acid Sequences.- Plant Transformation and Primary Analysis.- Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation.- Potato Transformation.- Transformation of Tomato.- Tobacco Transformation.- Genetic Transformation of Wheat.-Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309).- Molecular Analysis of Transgenic Rice.- PCR Analysis of Transgenic Tobacco Plants.- Southern Analysis of Transgenic Tobacco Plants.- Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes.- Western Analysis of Transgenic Plants.- Evaluation of Resistance.- Assaying Levels of Plant Virus by ELISA.- Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization.- Detection and Quantification of Plant Viruses by PCR.- Assaying Levels of Virus with Local Lesion Hosts.- Field Testing Resistance of Transgenic Plants.- Agronomic Performance of Transgenic Plants.- Mechanisms of Resistance.- Mechanisms of Resistance.- Mechanisms of RNA-Mediated Resistance to Plant Viruses.- Detection of Risks Associated with Coat Protein Transgenics.- Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom.
Synopsis
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
LC Classification Number
QK
Description de l'objet fournie par le vendeur
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Premier Books LLC
David Taylor
26C Trolley Sq
19806-3356 Wilmington, DE
United States
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- l***c (227)- Évaluations laissées par l'acheteur.Dernier moisAchat vérifiéBook came packaged well to avoid damage, in great condition. Not sure if the seller delayed shipping or if usps didn’t update the shipping, which is highly likely. Still received and we are happy with our purchase. I had trouble looking up tracking due to the company they used but seller found usps tracking and sent that over quickly. Sellers communication was excellent. Would buy from again. Item as described.
- t***n (2908)- Évaluations laissées par l'acheteur.Dernier moisAchat vérifiéI don't give negatives; However, description was not correct; No price guide was included in this book.As you will see in book pic shown; title states price guide included, no price guide inside. Communication poor, description, no communication price guide missing from this book. Shipping time was weeks before it was even shipped. Blamed the shipping on warehouse. You own & operate a business; your warehouse is not up to standards you change who you do business with. It's your responsibility.
- -***6 (133)- Évaluations laissées par l'acheteur.6 derniers moisAchat vérifiéItem arrived as described and adequately packaged. Ridiculously long shipping time. Seller posts incorrect tracking number and lists the item as shipped. Contacted seller for correct tracking, received a new tracking number with the warning that the number won’t be “live” until the item is shipped. Will you get your item? Yeah, probably. Count on it being delivered 2-3 weeks longer than expected.
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